Dietary fat supplementation relieves cold temperature-induced energy stress through AMPK-mediated mitochondrial homeostasis in pigs

Table 1 Ingredients and nutrient composition of the diets

Ingredients, %	Content, %
Ingredients, %	Basal diet	High-fat diet
Corn	72.30	62.50
Soybean meal	22.00	14.00
Full-fat soybean	0.00	15.00
Soybean oil	1.56	4.17
Dicalcium phosphate	1.20	1.34
Limestone	0.90	0.90
Salt	0.40	0.40
Lysine	0.43	0.45
Methionine	0.06	0.08
Threonine	0.12	0.13
Choline chloride	0.03	0.03
Premix¹	1.00	1.00
Total	100.0	100.0
Nutrient levels²
Metabolizable energy, Mcal/kg	3.20	3.42
Net energy, Mcal/kg	2.49	2.68
Crude fat	5.09	7.82
Crude protein	15.99	17.03
Lysine	0.98	1.06
Methionine	0.28	0.31
Threonine	0.60	0.63
Tryptophan	0.18	0.19
Calcium	0.66	0.71
Available phosphorus	0.26	0.28
Sodium	0.17	0.17
Chlorine	0.27	0.27

¹Provided the following per kilogram of diet: Fe, 140 mg; Cu, 25 mg; Mn, 35 mg; Zn, 80 mg; Se, 0.4 mg; I, 0.5 mg; vitamin A, 10,000 IU; vitamin D₃, 3,000 IU; vitamin E, 63 mg; vitamin K₃, 3 mg; vitamin B₁, 3 mg; vitamin B₂, 9.6 mg; vitamin B₆, 4.5 mg; vitamin B₁₂, 0.04 mg; niacin, 36 mg; d-pantothenic acid, 30 mg; d-biotin, 0.24 mg; and folate, 1.8 mg
²Crude fat and crude protein were analyzed values and the rest were calculated values

ISSN: 2049-1891