Fig. 6

Nrf2 inhibitor blocked the activation effect of hesperidin on the Keap1/Nrf2/ARE signaling pathway. A Protein levels of Nrf2 and β-actin by western blot analysis. B Quantitation of Nrf2 protein abundance. C Protein levels of Keap1, NQO1, HO-1 and Nrf2 by western blot analysis. D–G Quantitation of Keap1, NQO1, HO-1, and Nrf2 protein abundance. β-actin was used as an internal control. bMECs were treated with ML385 (5 μmol/L) for 48 h and/or H₂O₂ (1,000 μmol/L) for 8 h and/or hesperidin (120 μg/mL) for 24 h. All data are presented as the mean ± SEM from three independent experiments. ^*P < 0.05, ^**P < 0.01, and ^***P < 0.001