Fig. 5

Hesperidin induced the nuclear translocation of Nrf2 and activated the expression of antioxidant response genes in H₂O₂-treated bMECs. A Representative images of the cellular distribution of Nrf2, the scale bar represents 20 μm. B Representative western blots of Keap1, NQO1, HO-1, Nrf2, p38 MAPK, and β-actin was used as a loading control. C–G Quantitation of Keap1, NQO1, HO-1, Nrf2, and p38 MAPK protein abundance. bMECs were treated with H₂O₂ (1,000 μmol/L) for 8 h and/or hesperidin (120 μg/mL) for 24 h. All data are presented as the mean ± SEM from three independent experiments. ^*P < 0.05, ^**P < 0.01, and ^***P < 0.001