Fig. 4

Nlrc3 was the key factor to protect gut barrier integrity. A In vivo sinlrc3 interference for 1 d; B In vivo sinlrc3 interference for 7 d; C and F The relative gene expression of foxo3 and cylinD1 in the short-term and long-term experiments; D and G The relative gene expression of lgr5 and olfm4 in the short-term and long-term experiments; E and H The relative gene expression of zo-1 and ocludin in the short-term and long-term experiments (n = 5). Data are represented as mean ± SEM. Significant difference compared with siRNA-scramble group (Student’s t-test; ^**P < 0.01, ^***P < 0.001). CON, control; nlrc3, NLR family CARD domain containing 3; foxo3, forkhead box O3; lgr5, leucine-rich repeat-containing G protein-coupled receptor 5; olfm4, olfactomedin 4; zo-1, zona occludens 1