Fig. 3

Addition of P. pentosaceus YC inhibited Nlrc3 and promoted the gene expression of ISC markers. A KEGG enrichment analysis; B Heatmap of genes of NOD-like signaling pathway; C and D Volcano plot of DEGs in the compared groups (n = 3). E and F The relative gene expression of ISC markers (lgr5 and olfm4) in PI and DI; G The protein expression of Nlrc3 in PI and DI; H and I The quantification of the protein expression of Nlrc3 in PI and DI; J and K The relative gene expression of foxo3 and cyclinD1 in PI and DI (n = 6). Data are represented as mean ± SEM. Asterisk refers to the significant difference (ANOVA with Tukey’s test; ^*P < 0.05, ^**P < 0.01). CON, control diet; GOS, gossypol diet; GP, gossypol diet supplemented with P. pentosaceus YC; FC, fold change; lgr5, leucine-rich repeat-containing G protein-coupled receptor 5; olfm4, olfactomedin 4; nlrc3, NLR family CARD domain containing 3; foxo3, forkhead box O3; iκbκb, inhibitor of nuclear factor kappa B kinase; il-1β, interleukin-1β; PI, proximal intestine; DI, distal intestine