Table 2 Primer sequences used for real-time PCR analysis
Gene | Primer sequence (5′ → 3′) | Tm, °C | Amplicon size, bp | E-value, % | R2 | GenBank No. |
|---|---|---|---|---|---|---|
β-Actin | F: AAAGGGAAATCGTGCGTGAC R: AAGGAAGGCTGGAAGAGGG | 61 | 102 | 100.1 | 0.998 | XM_020154709.1 |
ACCA | F:CACCAGGAACATATCGGACA R: ATCAATGTCGCTGTCAGTCT | 63.0 | 184 | 99.8 | 0.996 | XM_038709737.1 |
FAS | F: TGATGATAACTGGCTTCGG R: TCAAACCTGGACCCTACCT | 60.6 | 86 | 100.1 | 0.998 | XM_038735140.1 |
SCD1 | F: CGCCGAAGATGATGGTGT R: GGAAGACTCGCAGTGGATTAG | 63.0 | 153 | 98.7 | 0.986 | XM_038735580.1 |
PPARγ | F: AGCAGACATCCGCCCTAA R: ACCTCGATCACGCCGTAC | 64.3 | 160 | 99.7 | 0.989 | XM_038695878.1 |
CPT1α | F: AGCCCCACCCCAACCTACCAG R: CGGCCCTCACGGAATAAACGC | 65.0 | 81 | 99.9 | 0.995 | XM_038695351.1 |
HSL | F: TACCCTCCGTGGCCTTTGACC R: CTCGTCTTCAGCCCCAGCGAT | 64.0 | 161 | 98.9 | 0.985 | XM_038725628.1 |
PPARα | F: CCACCGCAATGGTCGATATG R: TGCTGTTGATGGACTGGGAAA | 58.0 | 156 | 100.9 | 0.991 | XM_038738333.1 |
Beclin1 | F: AGCAGTAGACGGCTGTATGAA R: TCTGGAATGCTTCTGTATGGA | 59.5 | 120 | 100.1 | 0.995 | XM_038704178.1 |
ATG1 | F: ACCAGGACGCAAGTCACAC R: CAGCCTTGGCATCATAGTTT | 58.4 | 161 | 99.2 | 0.988 | XM_038704081.1 |
ATG101 | F: ATTGCCGCTCGGAAGTT R: ATCGCTGCCAGAGTTGCT | 58.4 | 275 | 98.5 | 0.995 | XM_038709111.1 |
ATG13 | F: GAAAGCAGGGACACGACTATG R: AACAACACCGACACGCACT | 59.0 | 104 | 99.1 | 0.985 | XM_038719443.1 |
ATG4b | F: CATACGACACACTTCGCTTTG R: CCATCTCCAGTCTCTGCCTAA | 59.0 | 275 | 97.4 | 0.989 | XM_038716733.1 |
ATG5 | F: CGTGGAGGAGATGTGGTTTG R: TGACGACTTGGCTCTTGTGC | 58.5 | 236 | 99.0 | 0.991 | XM_038710515.1 |
ATG7 | F: GACCCACTTGGTTTGCC R: GCGTCCCATATCTCCTTG | 60.2 | 182 | 100.3 | 0.998 | XM_038697785.1 |
ATG16 | F: CTGGCTGCCTCCAATGACTT R: ACCTTCCCACTGTGACCCGT | 58.4 | 186 | 102.5 | 0.987 | XM_038692419.1 |
LC3B | F: TAAAAACACAAGCGGA R: TTGGTAGTATGTAAACGG | 63.8 | 178 | 100.1 | 0.992 | XM_038705174.1 |
mcoln2 | F: GCTTCGTCATTCTTGTTTGCC R: CACACCGACCCACACCATT | 58.0 | 296 | 98.9 | 0.988 | XM_038715787.1 |
TFEB | F: GCCAACCTGACCATCAAACG R: CCACAGACGCCCGCAGTATA | 58.5 | 208 | 99.5 | 0.995 | XM_038696074.1 |
Lamp2 | F: TGGTCACTGTGGTGTCGTCG R: AGGTGTTCCTGGAGGGGTTG | 68.5 | 260 | 98.9 | 0.991 | XM_038699731.1 |
CAT | F: GTTCCCGTCCTTCATCCACT R: CAGGCTCCAGAAGTCCCACA | 60.4 | 142 | 100.2 | 0.996 | XM_038704976.1 |
GPX1α | F: CCCTGCAATCAGTTTGGACA R: TTGGTTCAAAGCCATTCCCT | 58.0 | 123 | 100.1 | 0.994 | XM_038697220.1 |
SOD1 | F: CCCCACAACAAGAATCATGC R: TCTCAGCCTTCTCGTGGA | 58.0 | 86 | 97.8 | 0.989 | XM_038708943.1 |
Keap1 | F: TATTTCCGTCAGTCCCTCAG R: GGCAGCCAGCAGTTGTTC | 63.0 | 215 | 99.4 | 0.990 | XM_038713667.1 |
Nrf2 | F: CTGGTCCGAGACATACGC R: CTCAGCAGACGCTCCTTC | 57.5 | 168 | 98.6 | 0.992 | XM_038720536.1 |
IL-1β | F: CGTGACTGACAGCAAAAAGAGG R: GATGCCCAGAGCCACAGTTC | 59.4 | 210 | 99.6 | 0.994 | XM_038733429.1 |
COX2 | F: CACTGGGTCGTGTCACTTT R: TGATTCTCCTCCTTGCTGT | 60.6 | 140 | 100.7 | 0.991 | YP_636060.1 |
Hepcidin1 | F: CATTCACCGGGGTGCAA R: CCTGATGTGATTTGGCATCATC | 58.0 | 140 | 100.1 | 0.997 | XM_038710826.1 |
NF-κB | F: TGATGATAACTGGCTTCGG R: TCAAACCTGGACCCTACCT | 60.6 | 178 | 99.8 | 0.992 | XM_038723088.1 |
IL-10 | F: CGGCACAGAAATCCCAGAGC R:CAGCAGGCTCACAAAATAAACATCT | 62.1 | 258 | 98.8 | 0.989 | XM_038696252.1 |
IKBα | F: CCCCAACTACAGTGGACAAA R: AAGGTCAAGGAGGCAACG | 55.6 | 158 | 99.7 | 0.994 | XM_038734994.1 |