Fig. 3

Biological pathways predicted as regulated by miRNAs up-regulated in OF-EVs and UF-EVs. A Pathways predicted as regulated by miRNA (bta-miR-148b) up-regulated in OF-EVs. B Pathways predicted as regulated by miRNAs (bta-miR-134, bta-miR-151-3p, bta-miR-155, bta-miR-188, bta-miR-181b, bta-miR-181d, bta-miR-224, bta-miR-23b-3p, bta-miR-24-3p, bta-miR-27a-3p, bta-miR-29a, bta-miR-324, bta-miR-326, bta-miR-345-3p, bta-miR-410, bta-miR-652, bta-miR-677, bta-miR-873 and bta-miR-708) up-regulated in UF-EVs. The left Y-axis values represent the percentage of genes predicted as modulated by the miRNAs for the respective pathways (number of genes predicted to be modulated by miRNAs in each group divided by the total number of genes of each pathway). The right Y-axis represents the enrichment score (P-value) for each interaction. Blue squares highlight functions with potential relevance for embryo development. Pathways were accessed through miRWalk 3.0 database