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Fig. 8 | Journal of Animal Science and Biotechnology

Fig. 8

From: High expression circRALGPS2 in atretic follicle induces chicken granulosa cell apoptosis and autophagy via encoding a new protein

Fig. 8

PARP1 promotes apoptosis and HMGB1 promotes autophagy in chicken granulosa cells. A Screening of different si-PARP1 fragments. B Screening of different si-HMGB1 fragments. C Flow cytometry results in granulosa cells after interference with PARP1. D Confocal microscopy observation of autophagosomes in granulosa cells after transfection with si-HMGB1. E The protein abundance and gray value statistics of caspase 3 and caspase 9 after transfection with si-PARP1. F The protein abundance and gray value statistics of Beclin 1 and LC3 after transfection with si-HMGB1. *P < 0.05, **P < 0.01. Data are presented as mean ± SEM. n = 9 for qRT-PCR, and n = 3 for Western blotting

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Journal of Animal Science and Biotechnology

ISSN: 2049-1891