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Fig. 5 | Journal of Animal Science and Biotechnology

Fig. 5

From: High expression circRALGPS2 in atretic follicle induces chicken granulosa cell apoptosis and autophagy via encoding a new protein

Fig. 5

Identification of circRALGPS2-212aa. A Schematic diagram of the construction of ov-circRALGPS2 and ov-circRALGPS2-FLAG. B Schematic diagram of the construction of pcDNA3.1-circRALGPS2-FLAG. C Levels of Flag-tagged protein and β-tubulin protein after transfection of ov-circRALGPS2-FLAG in granulosa cells. D Flag-tagged protein levels after transfection of pcDNA3.1-circRALGPS2-FLAG in granulosa cells. E Immunofluorescence of Flag-tagged after transfection of ov-circRALGPS2-FLAG in granulosa cells. F Western blot results of co-immunoprecipitation with FLAG antibody in granulosa cells. G Silver staining assay results after co-immunoprecipitation with FLAG antibody in granulosa cells. H LC-MS/MS analysis of circRALGPS2 peptides after co-immunoprecipitation. I LC-MS/MS analysis of inserted FLAG peptides and specific peptides of circRALGPS2

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Journal of Animal Science and Biotechnology

ISSN: 2049-1891