Fig. 3

Identification of circRALGPS2. A Schematic diagram of the source of circRALGPS2 and Sanger sequencing results. B Expression of circRALGPS2 in 10 different chicken tissues. C Expression levels of circRALGPS2 in the cytoplasm (red) and nucleus (blue) of granulosa cells. D Expression of linear RNA and circRALGPS2 after RNase R treatment. E qRT-PCR of linear RNA and circRALGPS2 from cDNA reverse transcribed from oligo-d(T)₁₈ and random primers. F Expression of circRALGPS2 after transfection with ov-circRALGPS2 plasmid. G Screening of different si-circRALGPS2 fragments. H FISH assay of circRALGPS2 in chicken granulosa cells. ^a–fBars with different letters indicate significant differences (P < 0.05), ^*P < 0.05, ^**P < 0.01. Data are presented as mean ± SEM. n = 9 for qRT-PCR