Fig. 2

CircRNA RNC-seq analysis of healthy and atretic follicles. A Schematic sequencing of healthy and atretic follicles. B Volcano plot of differentially expressed circRNAs in circRNA RNC-seq. C Volcano plot of differentially expressed circRNAs in circRNA RNA-seq. D Combined analysis of circRNA RNA-seq and RNC-seq. E Heatmap of 38 differentially expressed circRNAs in circRNA RNA-seq and RNC-seq. F Details of the top 5 circRNAs with the highest expression derived from exon splicing. G qRT-PCR validation of five dierentially expressed circRNA in healthy and atretic follicles. ^*P < 0.05, ^**P < 0.01. Data are presented as mean ± SEM. n = 9 for qRT-PCR