Fig. 1
From: tRNAGlu-derived fragments from embryonic extracellular vesicles modulate bovine embryo hatching
tsRNAs profiling of embryonic EVs derived from the conditioned medium of blastocysts (B) and non-blastocysts (NB). A sncRNAs mapping distribution of reads mapped to the Bos taurus genome (ARS-UCD1.2). B PCA of all 5 small RNA libraries based on tsRNAs expression. C Type percentages of tsRNAs. D Length distribution of reads mapped to tRNA. E Left: Heatmap showing the tsRNA relative expression levels (normalized to total miRNA levels and based on a Z-score transformed scale in the row direction) of five samples; Right: Percentage distribution of tsRNAs derived from the same tRNA. F Hierarchical clustering and heatmap of differentially expressed tsRNAs between B and NB. G A schematic diagram indicating the cleavage sites of tDR-14:32-Glu-CTC-1 from tRNAGlu-CTC. H Relative counts per million (CPM) fold change of tDR-14:32-Glu-CTC-1 in embryo derived EVs by small RNA-seq. I Relative expression level of tDR-14:32-Glu-CTC-1 in conditioned medium and embryos measured by RT-qPCR. Five biological replicates were used for RT-qPCR. Error bars represent SE. *P < 0.05