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Fig. 7 | Journal of Animal Science and Biotechnology

Fig. 7

From: Decreased eggshell strength caused by impairment of uterine calcium transport coincide with higher bone minerals and quality in aged laying hens

Fig. 7

Identification of apoptosis-related indicators at the initiation stage of eggshell calcification (8.5 h post-oviposition). The protein contents of p53 (A), p62 (B), LC3 (C), immunoglobulin A (IgA, D); the activity of caspase-8 and caspase-3 (E); Western blot results and quantification of Bax and Bcl-2 (F); TUNEL-positive cells ratio (G); TUNEL staining (20 ×) and HE staining (H), interstitial edemas (black arrow), loose connective tissue (red arrow) and inflammatory cell infiltration (yellow arrow). HBS, high eggshell breaking strength group; LBS, low eggshell breaking strength group. Asterisk (*) denotes significant difference (P < 0.05) between the HBS and LBS

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