Fig. 6

Functional enrichment analysis and gene network construction of DEGs at the initiation stage of eggshell calcification (8.5 h post-oviposition). Enrichment of DEGs that are active in regulation of cell killing, regulation of hemopoiesis, negative regulation of hemopoiesis, regulation of blood coagulation, negative regulation of cell activation, as well as positive regulation of immune effector process was noted. GO terms are presented as nodes in functionally grouped networks based on the GO cluster algorithm, where only the most significant term per group is labeled. The pie chart illustrates the percentage distribution of genes shown in the related functional groups