Fig. 3
OPLS-DA score scatter plots of liver metabolites and permutation test displaying the stability of the OPLS-DA model. A–D LC-MS. A OPLS-DA score scatter of the HELP group compared to the CT group, R2X = 0.792, R2Y = 0.870, Q2 = 0.0324. B OPLS-DA score scatter of the FRT4M group compared to the HELP group, R2X = 0.618, R2Y = 0.944, Q2 = 0.0962. C permutation test displaying the stability of the HELP group compared to the CT group, R2 = 0.785, Q2 = −0.509. D the FRT4M group compared to the HELP group, R2 = 0.878, Q2 = −0.293. E–H GC-MS. E OPLS-DA score scatter of the HELP group compared to the CT group, R2X = 0.423, R2Y = 0.998, Q2 = 0.539. F OPLS-DA score scatter of the FRT4M group compared to the HELP group, R2X = 0.483, R2Y = 0.992, Q2 = 0.373. G permutation test displaying the stability of the HELP group compared to the CT group, R2 = 0.986, Q2 = −0.096. H the FRT4M group compared to the HELP group, R2 = 0.983, Q2 = 0.101. CT: control group, hens fed with normal diet. HELP: model group, hens fed with high-energy low-protein diet. FRT4M: experimental group, hens fed with high-energy low-protein diet with 1010 CFU/kg Lp. plantarum FRT4