Supplementation of vitamin E or a botanical extract as antioxidants to improve growth performance and health of growing pigs housed under thermoneutral or heat-stressed conditions

Silva-Guillen, Ysenia V.; Arellano, Consuelo; Wiegert, Jeffrey; Boyd, R. Dean; Martínez, Gabriela E.; van Heugten, Eric

doi:10.1186/s40104-023-00981-7

Journal of Animal Science and Biotechnology

Table 6 Complete blood count measured on d 2 and d 28 in pigs exposed to thermo-neutral or heat-stressed environments and provided antioxidants in feed or water^a

From: Supplementation of vitamin E or a botanical extract as antioxidants to improve growth performance and health of growing pigs housed under thermoneutral or heat-stressed conditions

Item	Day	Environment^b								SEM	P-value^c
		Thermo-neutral				Heat-stressed					P-value^c
		CON	VEW	VEF	POL	CON	VEW	VEF	POL		E	S	D	E × D
White blood cells, 10³/μL	2	17.2	18.8	17.9	17.5	17.9	17.7	17.5	16.0	1.13	0.581	0.255	<0.001	0.840
White blood cells, 10³/μL	28	14.8	13.3	14.1	12.8	13.8	13.8	13.4	12.5	1.13	0.581	0.255	<0.001	0.840
Red blood cells, 10⁶/μL	2	6.65	6.83	6.71	6.51	6.84	6.76	6.73	6.58	0.22	0.287	0.750	0.430	0.020
Red blood cells, 10⁶/μL	28	6.94	6.94	6.80	7.33	6.57	6.70	6.42	6.56	0.22	0.287	0.750	0.430	0.020
Hemoglobin, g/dL	2	12.14	12.45	12.37	11.97	12.21	12.34	12.25	12.21	0.37	0.107	0.563	0.188	0.003
Hemoglobin, g/dL	28	12.73	12.98	12.79	13.49	11.72	12.09	11.98	11.99	0.37	0.107	0.563	0.188	0.003
Hematocrit, %	2	40.6	41.6	41.2	39.3	40.5	40.9	41.2	40.4	1.37	0.152	0.767	0.670	0.013
Hematocrit, %	28	42.0	42.1	41.8	44.8	39.0	40.2	38.5	39.5	1.37	0.152	0.767	0.670	0.013
Platelet Count, 10³/μL	2	252	260	242	247	312	270	305	282	31	0.268	0.542	<0.001	0.382
Platelet Count, 10³/μL	28	219	169	180	170	205	217	219	175	31	0.268	0.542	<0.001	0.382
Neutrophils, count/μL	2	7,223	6,559	6,353	6,401	6,471	6,430	5,969	5,221	572	0.247	0.215	<0.001	0.658
Neutrophils, count/μL	28	3,908	3,622	4,426	3,231	3,336	3,795	3,525	3,017	572	0.247	0.215	<0.001	0.658
Lymphocytes, count/μL	2	8,272	9,503	9,667	9,269	9,782	9,528	9,609	8,972	731	0.795	0.758	0.123	0.652
Lymphocytes, count/μL	28	9,786	8,540	8,462	8,396	9,164	8,850	8,676	8,415	731	0.795	0.758	0.123	0.652
Monocytes, count/μL	2	1,219	1,256	1,142	1,226	1,002	1,121	1,339	1,289	118	0.647	0.889	<0.001	0.768
Monocytes, count/μL	28	736	720	754	733	771	651	705	590	118	0.647	0.889	<0.001	0.768
Eosinophils, count/μL	2	400	435	653	519	582	531	499	468	99	0.500	0.702	0.304	0.336
Eosinophils, count/μL	28	348	412	458	422	534	535	502	497	99	0.500	0.702	0.304	0.336

^aValues are least square means of 16 pigs. Dietary treatments consisted of control diets (CON), vitamin E supplementation in water (VEW), vitamin E supplementation in feed (VEF), and botanical extract supplementation in feed (POL). Dietary and water treatments were provided starting on d −7 and environmental treatments started on d 0 until d 28
^bTemperatures were set at the following time points: 2400, 0200, 0400, 0600, 0800, 1000, 1200, 1400, 1600, 1800, 2000, and 2200 h. Temperatures for the thermo-neutral room were 18.9, 18.9, 20.0, 20.0, 21.1, 21.1, 22.2, 22.2, 21.1, 21.1, 20.0, and 20.0 °C, and for heat-stressed room they were 28.3, 29.4, 29.4, 31.1, 32.8, 33.3, 34.4, 35.6, 34.4, 31.7, 29.4 and 29.4 °C
^cEffects abbreviations: E = environment, S = supplementation, D = day, E × D = environment × day, E × S = environment × supplementation, S × D = supplementation × day, E × S × D = environment × supplementation × day. Interactive effects without significant differences or tendencies were not shown

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ISSN: 2049-1891

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