Establishment of a chicken intestinal organoid culture system to assess deoxynivalenol-induced damage of the intestinal barrier function

Table 1 List of primers

Genes	Description	Accession No.	Seqeunce (5'→3')
LGR5	Leucine rich repeat containing G protein-coupled receptor 5	XM_425441	GTG ACC AGT TGC CTA ATC TCC
LGR5	Leucine rich repeat containing G protein-coupled receptor 5	XM_425441	CGG AAT GTG TCT GCT TTG AT
MUC2	Mucin 2	XM_040701656	TGG TCT CTG TGG GGA TTA CA
MUC2	Mucin 2	XM_040701656	CCG AGT TTC ATC AGG GTC TT
CK19	Cytokeratin 19	NM_205009	CGT TGG AAG AAG CAA ACT CA
CK19	Cytokeratin 19	NM_205009	CGA TAG TGG CAG CAA GGA T
CHGA	Chromagranin A	XM_421330	ATT TCT ATC CTT CGC CAC CA
CHGA	Chromagranin A	XM_421330	TGC CAC ATC TCT TTG CTT GT
DCLK1	Doublecortin like kinase 1	NM_001257257	AAG CCT ACG GAA ACA GAG GA
DCLK1	Doublecortin like kinase 1	NM_001257257	TAT TGA TGC TGG AAC CTG GA
LYZ	Lysozyme	NM_205281	ACC CAG GCT ACA AAC CGT AA
LYZ	Lysozyme	NM_205281	CAG TTC ACG CTC GCT GTT AT
SLC7A5	Solute carrier familiy 7 member 5	NM_001030579.3	GGCTGTGGACTTTGGGAATC
SLC7A5	Solute carrier familiy 7 member 5	NM_001030579.3	AGAAGCCTCGGGTGAATCAT
GLUT1	Glucose transperter 1	NM_205209.2	CTGTTGCTGGGCTGTCTAAC
GLUT1	Glucose transperter 1	NM_205209.2	TCTCCGGCACCTTGAAGTAG
GAPDH	Glyceraldehyde-3-phosphate dehydrogenase	NM_204305	CCC CAA TGT CTC TGT TGT TG
GAPDH	Glyceraldehyde-3-phosphate dehydrogenase	NM_204305	GTG GAG GAA TGG CTG TCA C

ISSN: 2049-1891