Fig. 2

Characterization of chicken intestinal organoids. A Immunostaining of E-cadherin, villin, and CHGA of the chicken duodenum organoids at passage 1 and passage 10. B Chicken jejunum organoids were immunostained for E-cadherin, villin, and CHGA at passage 1 and passage 10. C Immunostaining of E-cadherin, villin, and CHGA of chicken ileum organoids at passage 1 and passage 10. Organoid immunostaining images were obtained under a confocal microscope. Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI; blue). Scale bar = 20 μm