Prevotella and succinate treatments altered gut microbiota, increased laying performance, and suppressed hepatic lipid accumulation in laying hens

Table 1 Composition and nutrient levels of experimental diets of laying hens

Ingredient, %	Content	Calculated nutrition levels, %	Content
Corn (8.5%)	58.41	Crude protein	16.50
Wheat bran	5.91	Metabolizable energy, kcal/kg	2,700
Soya-bean oil	1	Calcium	3.50
Soybean meal (46% CP)	22.68	Available phosphorus	0.404
Salt	0.35	Lysine	0.78
Limestone	9.61	Methionine	0.40
CaHPO₄	1.44	Threonine	0.505
Choline chloride, 50%	0.09	Tryptophan	0.161
DL-Methionine, 99%	0.16	Methionine + cystine	0.619
Lysine, 99%	0.10
Vitamin premix^a	0.05
Mineral premix^b	0.20

^aVitamin premix provides the following per kg of diet: VA, 8,800 IU; VD₃, 3,300 IU; VE, 16.5 IU; VK, 2.2 mg; VB₁, 1.7 mg; VB₂, 5.5 mg; VB₃, 6.6 mg; VB₅, 28 mg; VB₆, 3.3 mg; VB₇, 0.1 mg; VB₉, 0.6 mg; VB₁₂, 0.05 mg
^bMineral premix provides the following per kg of diet: Fe (as ferrous sulfate), 55 mg; Zn (as zinc sulfate), 88 mg; Mn (as manganese sulfate), 88 mg; Cu (as copper sulfate) 5.5 mg; I (as potassium iodide), 1.7 mg; Se (as sodium selenite), 0.3 mg

ISSN: 2049-1891