Table 2 Sequences of the oligonucleotide primers used for quantitative real-time PCR
Gene name | Primer sequence(5´→3´) | GenBank accession number |
|---|---|---|
GAPDH | F: AGAACATCATCCCAGCGTCC R: CGGCAGGTCAGGTCAACAAC | NM_204305 |
IL-1β | F: TGGGCATCAAGGGCTACA R: CGGCCCACGTAGTAAATGAT | NM_204524.1 |
TLR4 | F: GATGCATCCCCAGTCCGTG R: CCAGGGTGGTGTTTGGGATT | NM_001030693 |
NF-κB | F: TGGAGAAGGCTATGCAGCTT R: CATCCTGGACAGCAGTGAGA | NM_205134.1 |
IL-10 | F: CGCTGTCACCGCTTCTTCA R: TCCCGTTCTCATCCATCTTCTC | AJ621614 |
IFN-γ | F: AAAGCCGCACATCAAACACA R: GCCATCAGGAAGGTTGTTTTTC | NM_205149.1 |
ZO-1 | F: ACAGCTCATCACAGCCTCCT R: TGAAGGGCTTACAGGAATGG | XM_015278981.1 |
Claudin1 | F: GGTATGGCAACAGAGTGGCT R: CAGCCAATGAAGAGGGCTGA | NM_001013611 |
Occludin | F: AGTTCGACACCGACCTGAAG R: TCCTGGTATTGAGGGCTGTC | NM_205128.1 |