Abomasal infusion of branched-chain amino acids or branched-chain keto-acids alter lactation performance and liver triglycerides in fresh cows

Table 4 Lactation performance in multiparous Holstein fresh cows

Parameter	Treatments¹			SEM	P-value
Parameter	CON	BCA	BCK	SEM	Trt	Time	Trt × Time
DMI, kg/d	18.9	19.2	20.4	0.61	0.22	< 0.01	0.40
DMI, %BW	2.95	2.90	3.11	0.12	0.18	< 0.01	0.42
BW, kg	644	644	660	13.0	0.58	< 0.01	0.95
BW change, kg/week	–15.6	–17.0	–14.4	3.4	0.86	0.03	0.88
BCS	3.16	3.12	3.12	0.08	0.92	< 0.01	0.73
Milk composition, %
Fat	4.93	4.90	4.84	0.13	0.85	< 0.01	0.87
Protein	3.73^AB	3.51^B	3.83^A	0.08	0.01	< 0.01	0.85
Lactose	4.88	4.86	4.83	0.02	0.28	< 0.01	0.26
Solids not fat or protein	5.74	5.70	5.70	0.03	0.51	< 0.01	0.11
MUN², mg/dL	11.5^b	12.7^A,a	11.2^B	0.40	0.02	0.11	0.78
SCC³	1.55	1.52	1.64	0.07	0.46	< 0.01	0.42
Milk production, kg/d
Milk yield	35.3^B	39.5^A	35.1^B	1.80	0.02	< 0.01	0.34
Fat yield	1.69^B	2.10^A	1.67^B	0.08	0.02	0.02	0.72
Protein yield	1.25^b	1.46^a	1.30^b	0.07	0.10	0.17	0.80
Lactose yield	1.67^B	2.11^A	1.69^B	0.07	0.02	< 0.01	0.49
FCM³	41.1^B	52.0^A,a	42.0^b	3.3	0.04	< 0.01	0.50
ECM⁴	40.8^b	50.8^a	41.8^b	3.1	0.05	< 0.01	0.53
Milk:DMI	1.90^ab	2.11^a	1.79^b	0.08	0.03	< 0.01	0.23

^A,BWithin a row, means with different capitalized superscripts differ after controlling for pairwise LSM comparisons using Tukey’s post-hoc procedure (P ≤ 0.05)
^a,bWithin a row, means with different superscripts (lowercase) tend to differ after controlling for pairwise LSM comparisons using Tukey’s post-hoc procedure (0.05 < P ≤ 0.10)
¹CON Control (saline 0.9%), BCA Branched chain amino acids (67 g valine, 50 g leucine, and 34 g isoleucine), BCK Branched chain keto-acids (77 g keto-valine, 57 g keto-leucine, and 39 g keto-isoleucine)
²MUN Milk urea nitrogen
³Fat corrected milk calculated as FCM (kg) = 0.4324 × Milk yield (kg) + 16.216 × Fat yield (kg) [22, 23]
⁴Energy corrected milk calculated as ECM (kg) = 12.82 × fat yield (kg) + 7.13 × protein yield (kg) + 0.323 × milk yield (kg) [22, 23]

ISSN: 2049-1891