Abomasal infusion of branched-chain amino acids or branched-chain keto-acids alter lactation performance and liver triglycerides in fresh cows

Table 1 Ingredient and nutrient composition of the experimental diet fed to multiparous Holstein fresh cows

Item (DM basis)	Diet
Ingredient, %
Corn silage	34.8
Ground corn	18.5
Alfalfa silage	15.4
Soybean meal	10.2
Whole cottonseed	8.43
Alfalfa hay	5.62
Bypass protein^a	2.44
Mineral and vitamin premix^b	1.97
Calcium carbonate	1.19
Protein blend^c	1.14
Sodium sesquicarbonate	1.07
Urea	0.16
Rumen protected methionine^d	0.07
Tallow	0.01
Nutrient, mean ± SD^e
CP, %	17.5 ± 0.5
aNDFom^f	30.6 ± 0.8
ADF, %	20.9 ± 0.9
Lignin, %	3.6 ± 0.2
Starch, %	25.7 ± 1.3
Ether extract, %	4.3 ± 0.4
Ash, %	7.7 ± 1.1
Ca, %	1.17 ± 0.10
P, %	0.43 ± 0.02
Mg, %	0.30 ± 0.02
K, %	1.30 ± 0.07
Na, %	0.44 ± 0.03

^aAminoPlus, Ag Processing Inc., Omaha, NE, USA
^bThe premix contained 30.5% Na, 7,567 mg/kg Zn, 3,888 mg/kg Mn, 961 mg/kg Cu, 105 mg/kg Co, 125 mg/kg I, 67 mg/kg Cr, 74 mg/kg Se, 1,930 IU/g vitamin A, 1,110 IU/g vitamin D₃, and 29.66 IU/g vitamin E
^cCaledonia Pass, Caledonia Farmers Elevator Co.; 73% CP, a mix of mostly porcine blood meal, feather meal, and grain byproducts
^dSmartamine M, Adisseo, Alpharetta, GA, USA
^eMeans reported from composited weekly TMR samples (Cumberland Valley Analytical Services, Waynesboro, PA, USA)
^fAmylase-treated NDF

ISSN: 2049-1891