Table 2 Real time PCR primers
Gene | Accession No. | Primer | Sequence (5′→3′) | Product size, bp |
|---|---|---|---|---|
CaSR | XM_021068447.1 | Forward Reverse | TGCCCAGATGACTTCTGGTCC GCACGAGATGCAGAGCACGAAGC | 330 |
T1R1 | XM_013988748.1 | Forward Reverse | TACAACGGTCTCCTCTCGGT CAGCATGGCAAACACGTTGA | 192 |
T1R3 | NM_001113288.1 | Forward Reverse | TGTACCAGGTTCTCGTCCCT GGCCATGAACACTAGGCTG | 172 |
mGluR1 | XM_005659163.3 | Forward Reverse | GTCGGGAGCTCTGCTACATC GGCACTCATAAACCTGGGCT | 230 |
mGluR4 | XM_001928369.6 | Forward Reverse | GCCCAAGGTCTACGTCATCC CCTGGCTAGATCGCATGGTT | 228 |
SLC7A1 | NM_001012613.1 | Forward Reverse | TCTGGTCCTGGGCTTCATAA ACCTTCGTGGCATTGTTCAG | 123 |
SLC7A2 | NM_001110420.1 | Forward Reverse | GCAACAACTGGCGAAGAAGT GGCATCATAAGGGTCAAAGC | 122 |
GAPDH | NM_001206359.1 | Forward Reverse | TGGTGAAGGTCGGAGTGAAC GAAGGGGTCATTGATGGCGA | 104 |