Excess dietary Lys reduces feed intake, stimulates jejunal CCK secretion and alters essential and non-essential blood AA profile in pigs

Table 1 Composition of the experimental diets (as-fed basis)

Item	Dietary treatments¹
Item	T0	T1	T2	T3
Ingredients, %
Wheat	70.61	70.61	70.61	70.61
Soyabean meal	8.60	8.60	8.60	8.60
Full fat soya	4.80	4.80	4.80	4.80
Meat bone meal	5.35	5.35	5.35	5.35
Fish meal	5.00	5.00	5.00	5.00
Vegetable oil	3.00	3.00	3.00	3.00
Lysine HCl	0.46	0.77	1.23	1.98
DL-Methionine	0.10	0.10	0.10	0.10
Threonine	0.15	0.15	0.15	0.15
Tryptophan	0.01	0.01	0.01	0.01
L-Alanine	1.45	1.16	0.72	–
Salt	0.20	0.20	0.20	0.20
Vitamin and mineral premix²	0.20	0.20	0.20	0.20
Calculated nutrient content, %
Dry matter	90.61	90.61	90.61	90.61
Crude protein	20.60	20.60	20.60	20.60
Fat	6.10	6.10	6.10	6.10
Digestible energy, Mcal/kg	3.59	3.60	3.60	3.61
Calcium	0.96	0.96	0.96	0.96
Phosphorus	0.76	0.76	0.76	0.76
Lysine	1.29	1.53	1.88	2.48
SID Lys³	1.20	1.44	1.80	2.40
Methionine	0.42	0.42	0.42	0.42
Threonine	0.82	0.82	0.82	0.82
Tryptophan	0.22	0.22	0.22	0.22
Met/Lys	0.32	0.27	0.22	0.17
(Met + Cys)/Lys	0.56	0.47	0.38	0.29

¹T0 100% Lys requirement, T1 120% Lys requirement, T2 150% Lys requirement, T3 200% Lys requirement based on its standard ileal digestibility
²Provided the following (as-fed basis): vitamin A (10,000 IU/kg), vitamin D₃ (1,800 IU/kg), vitamin E (100 mg/kg), vitamin K₃ (5 mg/kg), vitamin B₁ (3 mg/kg), vitamin B₂ (6 mg/kg), niacin (30 mg/kg), pantothenic acid (30 mg/kg), pyridoxine (4 mg/kg), biotin (0.3 mg/kg), folic acid (2.5 mg/kg), vitamin B₁₂ (0.04 mg/kg), iron (100 mg/kg), iodine (0.7 mg/kg), manganese (45 mg/kg), selenium (0.3 mg/kg), zinc (120 mg/kg), cobalt (0.3 mg/kg), copper (10 mg/kg)
³Standard ileal digestible lysine

ISSN: 2049-1891