The chemical characteristics of different sodium iron ethylenediaminetetraacetate sources and their relative bioavailabilities for broilers fed with a conventional corn-soybean meal diet

Table 9 Effects of dietary Fe on protein levels of liver Fe-containing enzymes of broilers on d 21

Fe source	Added Fe, mg/kg	CAT⁴, RQ	SDH⁴, RQ	COX⁴, RQ
Control¹	0	0.85	0.91	0.76*
FeSO₄∙7H₂O¹	20	0.88	0.91	0.82
	40	0.96	0.90	0.92
	60	0.98	0.88	0.94
Fe-Prot ES¹	20	0.83	0.84	0.93
	40	1.08	0.91	1.01
	60	0.83	0.77	0.92
Feed grade NaFeEDTA¹	20	0.98	0.91	0.95
	40	0.94	0.93	0.86
	60	0.90	1.02	0.90
Food grade NaFeEDTA¹	20	0.89	1.08	1.03
	40	0.91	0.99	1.02
	60	0.83	0.94	0.93
Pooled SE		0.12	0.09	0.08
Fe source²	FeSO₄∙7H₂O	0.94	0.90	0.90
	Fe-Prot ES	0.91	0.84	0.95
	Feed grade NaFeEDTA	0.94	0.95	0.88
	Food grade NaFeEDTA	0.88	1.00	0.99
Pooled SE		0.07	0.05	0.05
Added Fe level³, mg/kg	20	0.90	0.93	0.93
	40	0.97	0.93	0.95
	60	0.89	0.90	0.90
Pooled SE		0.06	0.05	0.04
P-value	Fe source	0.9043	0.1428	0.3103
	Added Fe level	0.5165	0.8610	0.6673
	Fe Source × added Fe level	0.8383	0.7942	0.7392

CAT Catalase, SDH Succinate dehydrogenase, COX Cytochrome c oxidase
¹Data represent the means of 7 replicate cages (n = 7)
²Data represent the means of 21 replicate cages (n = 21)
³Data represent the means of 28 replicate cages (n = 28)
⁴The protein expression levels were calculated as the relative quantities (RQ) of the target gene protein to the GAPDH protein
*Different (P < 0.05) from all Fe supplemental groups

ISSN: 2049-1891