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Table 7 Effects of dietary Fe on activities of tissue Fe-containing enzymes of broilers on d 21

From: The chemical characteristics of different sodium iron ethylenediaminetetraacetate sources and their relative bioavailabilities for broilers fed with a conventional corn-soybean meal diet

Fe source

Added Fe,

mg/kg

CAT activity,

U/mg protein

SDH activity,

U/mg protein

COX activity, mU/mg protein

Heart

Kidney

Liver

Heart

Kidney

Liver

Liver

Control1

0

2.21

19.0

16.2*

3.44*

4.34*

2.33*

28.7*

FeSO4∙7H2O1

20

2.23

22.9

16.5

3.57

4.43

2.37

30.1

40

2.13

20.1

16.6

3.96

4.81

2.57

31.6

60

2.19

20.6

16.7

4.12

4.92

2.81

34.9

Fe-Prot ES1

20

2.07

20.6

16.9

3.63

4.72

2.43

29.9

40

2.25

20.8

17.4

3.99

5.28

2.69

34.5

60

2.07

18.6

18.2

4.41

5.45

2.91

35.5

Feed grade NaFeEDTA1

20

2.02

19.5

16.7

3.95

5.32

2.60

29.1

40

2.08

20.5

17.4

4.40

5.51

2.78

34.5

60

2.15

19.4

18.4

4.62

6.22

2.82

35.3

Food grade

NaFeEDTA1

20

2.08

18.5

17.4

4.19

4.98

2.64

31.1

40

1.92

18.9

17.9

4.47

5.58

2.79

33.7

60

2.00

20.2

19.0

4.65

6.46

2.86

36.0

Pooled SE

 

0.18

1.40

0.41

0.31

0.36

0.17

1.71

Fe source2

FeSO4∙7H2O

2.18

21.2

16.6B

3.88

4.72B

2.58

32.2

Fe-Prot ES

2.13

19.8

17.5A

4.00

5.15AB

2.68

33.3

Feed grade NaFeEDTA

2.08

19.8

17.5A

4.32

5.68A

2.73

33.0

Food grade NaFeEDTA

2.00

19.2

18.1A

4.44

5.67A

2.76

33.6

Pooled SE

 

0.10

0.81

0.24

0.19

0.21

0.10

1.01

Added Fe level3, mg/kg

20

2.10

20.4

16.9b

3.83b

4.86b

2.51b

30.1b

40

2.09

19.9

17.3b

4.20ab

5.29ab

2.71ab

33.6a

60

2.10

19.7

18.1a

4.45a

5.76a

2.85a

35.4a

Pooled SE

 

0.09

0.70

0.21

0.17

0.18

0.09

0.86

P-value

Fe source

0.6307

0.3541

0.0006

0.1672

0.0052

0.5973

0.7885

Added Fe level

0.9974

0.7886

0.0011

0.0480

0.0049

0.0244

0.0002

Fe Source × added Fe level

0.9529

0.6721

0.7452

0.9993

0.8438

0.9812

0.9456

Linear effect4

–

–

0.0002

0.0029

0.0006

0.0020

< 0.0001

  1. CAT Catalase, SDH Succinate dehydrogenase, COX Cytochrome c oxidase
  2. 1Data represent the means of 7 replicate cages (n = 7)
  3. 2Data represent the means of 21 replicate cages (n = 21)
  4. 3Data represent the means of 28 replicate cages (n = 28)
  5. 4Linear effects of added Fe levels
  6. A,BMeans with different superscripts between Fe sources differ (P < 0.05)
  7. a,bMeans with different superscripts between added Fe levels differ (P < 0.05)
  8. *Different (P < 0.05) from all Fe supplemental groups