Skip to main content

Table 10 Multiple linear regressions of dependent variables on daily dietary analyzed Fe intake1

From: The chemical characteristics of different sodium iron ethylenediaminetetraacetate sources and their relative bioavailabilities for broilers fed with a conventional corn-soybean meal diet

Dependent variable

Regression equation2

R2

P-value

PI

Y = 0.6261 + 0.1137X1 + 0.1084X2 + 0.1216X3 + 0.1368X4

0.42

< 0.0001

TS

Y = 14.1258 + 4.5054X1 + 4.2214X2 + 4.562X3 + 4.9182X4

0.35

< 0.0001

Liver Fe

Y = 67.092 + 5.821X1 + 9.228X2 + 10.414X3 + 10.883X4

0.34

< 0.0001

Liver SDH activity

Y = 2.0216 + 0.1080X1 + 0.1310X2 + 0.1365X3 + 0.1465X4

0.12

0.0329

Heart SDH activity

Y = 2.5816 + 0.2538X1 + 0.2834X2 + 0.3371X3 + 0.3653X4

0.22

0.001

Kidney SDH activity

Y = 3.4912 + 0.2365X1 + 0.3272X2 + 0.4243X3 + 0.4378X4

0.28

< 0.0001

Liver CAT activity

Y = 14.9025 + 0.3275X1 + 0.5077X2 + 0.5121X3 + 0.6353X4

0.37

< 0.0001

Liver COX activity

Y = 20.679 + 2.236X1 + 2.450X2 + 2.410X3 + 2.572X4

0.25

< 0.0001

Liver SDH mRNA

Y = 0.7122 + 0.0604X1 + 0.0865X2 + 0.09490X3 + 0.0978X4

0.26

< 0.0001

Kidney SDH mRNA

Y = 0.8306 + 0.0537X1 + 0.0924X2 + 0.0943X3 + 0.0882X4

0.29

< 0.0001

  1. PI Plasma iron, TS Transferrin saturation, CAT Catalase, SDH Succinate dehydrogenase, COX Cytochrome c oxidase
  2. 1Daily dietary analyzed Fe intake = average daily feed intake during 1–21 days of age times the dietary analyzed Fe content for each respective Fe source. Regression analyses of PI, TS in plasma, liver Fe contents, tissue CAT, COX and SDH activities, and SDH mRNA expression levels were based on cage averages with 21 cages (3 chicks killed/cage) per Fe source
  3. 2Y is the PI (μg/mL), TS in plasma (%), tissue SDH and CAT enzyme activities (U/mg protein), liver Fe (μg/g, fresh basis), and SDH mRNA expression levels (RQ) in liver and kidney; X1 is the daily dietary analyzed Fe intake (mg) for FeSO4∙7H2O; X2 is the daily dietary analyzed Fe intake (mg) for Fe-Prot ES; X3 is the daily dietary analyzed Fe intake (mg) for feed grade NaFeEDTA; X4 is the daily dietary analyzed Fe intake (mg) for food grade NaFeEDTA