Table 1 Primer sequences for real-time PCR
Gene name | Sequence (5′→3′) | Product size, bp |
|---|---|---|
MHC I | F: AAGAACCTGCTGCGGCTG | 250 |
R: CCAAGATGTGGCACGGCT | ||
MHC IIa | F: GAGGAACAATCCAATACAAATCTATCT | 173 |
R: CCCATAGCATCAGGACACGA | ||
MHC IIb | F: GACAACTCCTCTCGCTTTGG | 274 |
R: GGACTGTGATCTCCCCTTGA | ||
MHC IIx | F: GGAGGAACAATCCAATGTCAAC | 178 |
R: GTCACTTTTTAGCATTTGGATGAGTTA | ||
PGC-1α | F: TATTTGCATCCAGAGCATGGC | 181 |
R: CCAGAGCAGCACAAAAGTACC | ||
NRF1 | F: CATGGCACTCAACAGCGAAG | 177 |
R: GGGGTCTTCCAGAATTGGGT | ||
TFAM | F: AGGATGGCACATCACAGGTAA | 168 |
R: GGTCTTCTCGTCCAACTTCCA | ||
UQCRC1 | F: TGGCTGGATTTGGCCCCATT | 136 |
R: TTTATGGTGGAGGGACGGCG | ||
ATP5A1 | F: CTTCAGAAAACCGGCACTGCT | 210 |
R: GTCAGGCTCCAAGTTCAGAGAC | ||
β-actin | F: CGGCTTTCGGTTGAGCTGAC | 159 |
R: GCCGTACCCACCAGAGTGAA |