Fig. 3

Effects of rumen microbiota transplantation (FR), sterile rumen fluid transplantation (SR) on production performance, plasma lipidome in postpartum dairy cows. The dairy cows in the CON group received 10 L saline daily. A–C Average dry matter intake (A), milk production (B), and feed efficiency (C) at 3-d intervals during postpartum 21 d including 7-time points. D and E The lipidome profiles of plasma visualized by score plots of principal component analysis (PCA) in the positive model (D) and negative model (E). F Heatmap plot used to show the relative intensive of SDL with FC > 2 or < 0.5. The colors of heatmap cells indicate the level of lipids across different samples. G and H Mantel analysis between significantly different lipids matrix and plasma parameter matrix. Color gradients indicate Spearman’s correlation coefficients. Edge width corresponds to the Mantel’s r value and edge color denotes the statistical significance. ^*0.01 ≤ P < 0.05; ^**0.001 ≤ P < 0.01; ^***0.0001 ≤ P < 0.001. Data are shown as mean ± SEM. n = 10