Dietary supplemental coated essential oils and organic acids mixture improves growth performance and gut health along with reduces Salmonella load of broiler chickens infected with Salmonella Enteritidis

Table 7 Effect of dietary EOA on mRNA expression of ileal inflammatory genes of broiler chickens infected with Salmonella Enteritidis (n = 6)

Items	Groups					SEM¹	P-value
Items	A	B	BL	BM	BH	SEM¹	P1²	Linear³	Quadratic⁴
3 DPI
TLR4	0.76	1.00	0.66	0.65	0.74	0.062	0.418	0.086	0.363
NF-κB	0.68^ab	1.00^a	0.58^ab	0.51^b	0.76^ab	0.064	0.129	0.066	0.139
MyD88	1.08	1.00	0.96	0.81	0.89	0.063	0.711	0.509	0.973
IL-6	0.63	1.00	0.50	0.33	0.64	0.115	0.462	0.167	0.418
IL-1β	0.69^ab	1.00^a	0.56^ab	0.42^b	0.74^ab	0.069	0.078	0.046	0.137
TNF-a	0.66^ab	1.00^a	0.94^a	0.55^b	0.92^a	0.059	0.043	0.183	0.621
IFN-γ	0.88	1.00	0.50	0.33	0.64	0.094	0.281	0.597	0.822
10 DPI
TLR4	0.89^ab	1.00^a	0.48^c	0.66^bc	0.36^c	0.066	0.002	0.001	0.559
NF-κB	0.94^b	1.00^b	1.47^a	1.20^ab	0.96^b	0.058	0.027	0.941	0.005
MyD88	1.35^b	1.00^b	2.69^a	2.89^a	1.35^b	0.206	0.001	0.091	0.001
IL-6	0.86^c	1.00^c	2.59^a	2.32^b	1.70^b	0.175	< 0.001	0.003	0.002
IL-1β	0.72	1.00	1.07	1.10	0.92	0.071	0.413	0.871	0.470
TNF-a	0.95	1.00	0.94	0.94	0.50	0.071	0.189	0.108	0.136
IFN-γ	0.55	1.00	0.94	1.06	1.15	0.101	0.330	0.711	0.648

¹SEM Standard error of the mean
²P1-value represent the difference comparison between group A, B, BL, BM and BH groups
³Linear regression analysis among B, BL, BM and BH groups
⁴Quadratic curve analysis among B, BL, BM and BH groups
^a–cMeans within the same row without a common superscript differ significantly (P < 0.05)
DPI: days post infection, A: broiler chickens with basal diet and no infection, B: broiler chickens with basal diet and SE infection, BL: broiler chickens with basal diet supplemented with 300 mg/kg EOA and SE infection, BM: broiler chickens with basal diet supplemented with 500 mg/kg EOA and SE infection, BH: broiler chickens with basal diet supplemented with 800 mg/kg EOA and SE infection. EOA: coated essential oils and organic acids mixture

ISSN: 2049-1891