Dietary supplemental coated essential oils and organic acids mixture improves growth performance and gut health along with reduces Salmonella load of broiler chickens infected with Salmonella Enteritidis

Table 6 Effect of dietary EOA on mRNA expression of ileal tight junction proteins of broiler chickens infected with Salmonella Enteritidis (n = 6)

Items	Groups					SEM¹	P-value
Items	A	B	BL	BM	BH	SEM¹	P1²	Linear³	Quadratic⁴
3 DPI
CLDN-1	2.72^a	1.00^b	1.35^b	1.17^b	1.03^b	0.157	0.001	0.679	0.255
OCLN	1.83^a	1.00^b	1.05^b	1.07^b	0.60^b	0.109	0.002	0.372	0.100
ZO-1	3.18^a	1.00^b	1.41^b	1.30^b	1.01^b	0.193	0.001	0.356	0.025
MUC-2	1.83^a	1.00^b	1.22^b	1.31^b	1.19^b	0.088	0.022	0.180	0.571
FABP-2	1.46	1.00	1.26	1.17	1.44	0.090	0.481	0.219	0.743
10 DPI
CLDN-1	2.25^bc	1.00^d	2.46^b	3.49^a	1.68^c	0.207	0.001	< 0.001	< 0.001
OCLN	1.80^b	1.00^c	2.78^a	2.98^a	1.32^bc	0.188	< 0.001	0.002	< 0.001
ZO-1	1.32^bc	1.00^c	1.84^a	1.70^ab	1.66^ab	0.083	0.002	0.001	0.033
MUC-2	3.17^b	1.00^d	4.99^a	5.69^a	2.07^c	0.393	< 0.001	0.002	< 0.001
FABP-2	1.61^dc	1.00^d	5.68^a	4.04^b	1.93^c	0.417	< 0.001	< 0.001	< 0.001

¹SEM Standard error of the mean
²P1-value represent the difference comparison between group A, B, BL, BM and BH groups
³Linear regression analysis among B, BL, BM and BH groups
⁴Quadratic curve analysis among B, BL, BM and BH groups
^a–dMeans within the same row without a common superscript differ significantly (P < 0.05)
DPI: days post infection, A: broiler chickens with basal diet and no infection, B: broiler chickens with basal diet and SE infection, BL: broiler chickens with basal diet supplemented with 300 mg/kg EOA and SE infection, BM: broiler chickens with basal diet supplemented with 500 mg/kg EOA and SE infection, BH: broiler chickens with basal diet supplemented with 800 mg/kg EOA and SE infection. EOA: coated essential oils and organic acids mixture

ISSN: 2049-1891