Dietary supplemental coated essential oils and organic acids mixture improves growth performance and gut health along with reduces Salmonella load of broiler chickens infected with Salmonella Enteritidis

Table 4 Effects of dietary EOA supplementation on ileal morphology of broiler chickens infected with Salmonella Enteritidis (n = 6)

Items	Groups					SEM¹	P-value
Items	A	B	BL	BM	BH	SEM¹	P1²	Linear³	Quadratic⁴
3 DPI
Villus height, μm	621.18	539.93	594.39	590.57	580.15	10.236	0.107	0.067	0.209
Crypt depth, μm	101.08	105.56	105.10	95.26	100.29	1.919	0.477	0.183	0.920
VH/CD	6.20^a	5.15^b	5.70^ab	6.21^a	5.79^ab	0.132	0.040	0.012	0.326
10 DPI
Villus height, μm	699.76	695.11	672.05	761.26	696.20	19.022	0.714	0.650	0.982
Crypt depth, μm	104.75^b	139.75^a	109.94^b	111.91^b	109.27^b	3.435	0.001	0.001	0.162
VH/CD	6.73^a	5.11^b	6.11^ab	6.77^a	6.36^ab	0.209	0.043	0.003	0.364

¹SEM Standard error of the mean
²P1-value represent the difference comparison between group A, B, BL, BM and BH groups
³Linear regression analysis among B, BL, BM and BH groups
⁴Quadratic curve analysis among B, BL, BM and BH groups
^a,bMeans within the same row without a common superscript differ significantly (P < 0.05)
DPI: days post infection, VH/CD: villus height to crypt depth ratio. A: broiler chickens with basal diet and no infection, B: broiler chickens with basal diet and SE infection, BL: broiler chickens with basal diet supplemented with 300 mg/kg EOA and SE infection, BM: broiler chickens with basal diet supplemented with 500 mg/kg EOA and SE infection, BH: broiler chickens with basal diet supplemented with 800 mg/kg EOA and SE infection. EOA: coated essential oils and organic acids mixture

ISSN: 2049-1891