Table 2 Sequences of the oligonucleotide primers used for quantitative real-time PCRa
Gene | Primer sequence (5′→3′) | GenBank ID |
|---|---|---|
Barrier-related genes | ||
 CLDN-1 | F: CATACTCCTGGGTCTGGTTGGT | NM_001013611.2 |
R: GACAGCCATCCGCATCTTCT | ||
 OCLN | F: ACGGCAGCACCTACCTCAA | NM_205128.1 |
R: GGGCGAAGAAGCAGATGAG | ||
 ZO-1 | F: CTTCAGGTGTTTCTCTTCCTCCTC | XM_040706827.1 |
R: CTGTGGTTTCATGGCTGGATC | ||
 MUC-2 | F: TTCATGATGCCTGCTCTTGTG | XM_421035 |
R: CCTGAGCCTTGGTACATTCTTGT | ||
 FABP-2 | F: GAAGCAATGGGCGTGAATGTGATG | NM_001007923.1 |
R: TTCGATGTCGATGGTACGGAAGTTG | ||
Immune-related genes | ||
 NF-κB | F: TGGAGAAGGCTATGCAGCTT | NM_205134.1 |
R: CATCCTGGACAGCAGTGAGA | ||
 TLR-4 | F: CCACTATTCGGTTGGTGGAC | NM_001030693.1 |
R: ACAGCTTCTCAGCAGGCAAT | ||
 MyD88 | F: TGCAAGACCA TGAAGAACGA | NM_001030962.3 |
R: TCACGGCAGCAAGAGAGATT | ||
 IL-6 | F: GATCCGGCAGATGGTGATAA | NM_204628.1 |
R: AGGATGAGGTGCATGGTGAT | ||
 IL-1β | F: TCATCTTCTACCGCCTGGAC | NM_204524.1 |
R: GTAGGTGGCGATGTTGACCT | ||
 TNF-α | F: GAGCGTTGACTTGGCTGTC | NM_204267.1 |
R: AAGCAACAACCAGCTATGCAC | ||
 IFN-γ | F: CTTCCTGATGGCGTGAAGA | NM_205149.1 |
R: GAGGATCCACCAGCTTCTGT | ||
 β-actin | R: GAGAAATTGTGCGTGACATCA | NM_205518.1 |
F: CCTGAACCTCTCATTGCCA | ||