Fig. 8

Candidate discriminant biomarkers of S. uberis-positive cows and healthy cows. (A) A circus plot showing the correlation between candidate biomarkers, including 6 DE genes (blue), 5 dMHBs (red), and 15 DMCs (in CpG context) (green). The external lines display the relative expression/methylation levels of selected candidates with respect to each outcome category. The yellow and blue lines represent the gene expression/methylation levels of S. uberis-positive and health control groups respectively, and the outer line represents the higher level. (B) Cluster of candidate biomarkers. Samples are represented in rows, while candidate biomarkers are represented in columns. (C-E) The loading plots of DE genes (C), dMHBs (D), and DMCs (CpG context) (E). The color of bar represents the group where the mean expression/methylation level is maximal. Orange and blue represent S. uberis-positive and healthy groups, respectively. The x-axis represents the loading weights, the negative value (orange bar) means the corresponding biomarker had higher expression/methylation level in S. uberis-positive group, while the positive value with blue bar means higher expression/methylation level in healthy control group