Fig. 7From: Dietary supplementation of Macleaya cordata extract and Bacillus in combination improve laying performance by regulating reproductive hormones, intestinal microbiota and barrier function of laying hensEffects of dietary supplementation with different biological feed additives on the Intestinal physical barrier function. (A) Histomorphology of the jejunum in laying hens. (B) The relative mRNA expressions of ZO-1, Claudin-1, Occludin and MUC-2 were analyzed by real-time qPCR. Data are presented as means ± SEM (n = 3). Different lowercase letters indicate a significant difference (P < 0.05). ZO-1: zonula occluden 1; MUC-2: mucin-2. (C) Transmission electron micrographs of the jejunum microvilli in laying hensBack to article page