Fig. 7From: Differential transcriptional regulation of the NANOG gene in chicken primordial germ cells and embryonic stem cellsRelative gene expression analysis after knockdown of predicted transcription factors (TFs) in cultured primordial germ cells (PGCs) and embryonic stem cells (ESCs). a Efficiency of small interfering RNA (siRNA)-mediated knockdown of TP53 in PGCs was analyzed by quantitative reverse-transcription PCR (RT-qPCR). Relative expression of NANOG was determined. b–f Efficiency of siRNA-mediated knockdown of CEBPA, CEBPB, CEBPD, CEBPG, and CEBPZ in ESCs was analyzed by RT-qPCR. Relative expression of NANOG was determined in each sample. Error bars indicate the standard deviation of triplicate analyses. Significant differences are indicated as * P < 0.05, ** P < 0.01, and *** P < 0.001Back to article page