Fig. 5From: Differential transcriptional regulation of the NANOG gene in chicken primordial germ cells and embryonic stem cellsVerification of the proximal enhancer of the chicken NANOG (cNANOG) gene in chicken embryonic stem cells (ESCs). a-b Schematic diagram of the constructed cNANOG promoter vectors and luciferase activity in ESCs (a) and DF-1 cells (b). c Prediction of transcription factor (TF)-binding sites in the cNANOG promoter region from − 442 to − 250 bp. d Mutation analysis of putative CCAAT/enhancer-binding protein (CEBP)-binding sites in ESCs. e Luciferase activity of the − 442/+ 70 bp cNANOG promoter fragment compared with that of the mutated promoter. Significant differences are indicated as ns (no significance) and *** P < 0.001. Error bar represent the SEs for five replicate reactionsBack to article page